Cloning and Sequence Analysis of LFYZQ from Brassica oleracea L. var. capitata L.
TANG Qing-Lin, WANG Zhi-Min, REN Xue-song, SONG Ming*, WANG Xiao-Jia
College of Horticulture and Landscape Architecture, Southwest University, Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China
Abstract：DNA and RNA were extracted from bolting stem apex of cabbage（Brassica oleracea L. var. capitata L.）cultivar ZQ and LFYZQ gene fragments were cloned by RT-PCR respectively with 2 pairs of primers. LFYZQ of DNA and cDNA sequence fragments assembly were respectively 2 560 bp and 1 239 bp. Analysis of the nucleotide sequence by BLAST on line indicated that homology of LFYZQ was up to 91 % between Brassica oleracea L. var. capitata L. and Brassica oleracea L. var. botrytis L.，up to 87 % between it and Arabidopsis thaliana, up to 86 % between it and Brassica juncea Coss., and up to 87 % between it and Raphanus sativus L. Analysis of gene structure by DNAstar indicated that LFYZQ including 3 extrons（452, 394, 393 bp）which totally coded 412 amino acids and 2 introns（514, 807 bp）which were unanimous with nucleotide splicing rules of GT-AG. Analysis of amino acids among LFYZQ and other 12 kinds of Brassicaceae LFY on line were divided into 2 classes: class Ⅰ with 3 plants（Brassica oleracea var. botrytis L., Brassica oleracea var. capitata L., Jonopsidium acaule）and class Ⅱ with other 10 plants. Molecular relativity quality of LFYZQ was 46 kD. LFYZQ was an unstable and hydrophobic protein with 4 kinds of active sites: N-myristoylation site, protein kinase C phosphorylation site, Casein kinase Ⅱ phosphorylation site and Amidation site.
