1Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, Fujian, China; 2Fuzhou Institute of Agricultural Sciences, Fuzhou 350018, Fujian, China
Abstract：The geographic divergence of the Lagenaria siceraria ssp. asiatica were examined using ITS1+5.8S rDNA+ITS2 sequences data. The sequences were obtained from 23 species of L. siceraria ssp. asiatica from China, Thailand and Japan. The results showed that within L. siceraria ssp. asiatica, ITS1+5.8S rDNA+ITS2 was 611-627 bp, the G+C content of the sequences was 54.17 %- 63.26 %, 65 informative sites（11.09 %）within L. siceraria ssp. asiatica are found in the ITS1+5.8S rDNA+ITS2 sequences. The length of the ITS1+5.8S rDNA+ITS2 sequences of 2 species from Japan is shorter than the other 21 species from China and Thailand, the G+C content of the sequences of the 2 species from Japan is lower than those from China and Thailand. Finally we constructed the strict consensus tree of 23 species of L. siceraria ssp. asiatica indicated that L. siceraria ssp. asiatica has two main prophylactic glades, one including 2 species from Japan, and the other including 20 species from China and 1 species from Thailand. The bootstrap value is 99 %. Test of the homogeneity of substitution patterns revealed that the species of L. siceraria ssp. asiatica from Japan are significantly different from those from China and Thailand.
