1College of Agronomy and Biotechnology，Yunnan Agricultural University，Kunming 650201，Yunnan，China|2Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China|3Institute of Horticulture，Yunnan Academy of Agricultural Sciences，Kunming 650205，Yunnan，China|4Northeast Agricultural University，Harbin 150030，Heilongjiang，China
摘要 为了研究白菜类作物氮利用效率遗传机制，定位与氮利用效率紧密相关的标记位点，利用分布于白菜全基因组的207个InDel标记，对160份有代表性的白菜类作物自然群体的基因组变异进行扫描，采用混合模型分析该群体的遗传结构，并应用TASSEL软件的GLM（General Linear Model）程序对叶片形态、植株形态和生物量相关的10个性状分别在两个氮处理水平进行关联分析。结果表明：InDel数据遗传结构分析将160个单株分为5个亚群体。关联分析结果表明：云南和北京的试验分别检测出45个和35个显著性相关标记，其中4个标记为两年共定位，平均贡献率为0.06。值得注意的是，关联到86个显著性变异位点只在一个氮水平被检测到，可能是氮处理水平的变化影响了某些相关基因的表达调控。
Abstract：In order to illustrate the genetic mechanism of nitrogen use efficiency（NUE）and identify the gene-linked markers for NUE in Brassica campestris L.，we genotyped 160 representative B. campestris accessions using 207 Insertion/Deletion（InDel）locus evenly distributed along the genome. Population structure was firstly analyzed for the population，then the structure-based association analysis between InDel locus and 10 leaf morphology and biomass traits in two contrasting N environments was performed by using TASSEL GLM（general linear model）program. Genetic structure analysis showed that 160 lines were composed of five subpopulations. We identified 45 and 35 markers significantly associated with 10 traits in experiments performed in Yunnan and Beijing，respectively. Among those，4 markers were co-localized in two experiments and the loci identified explained 0.06 of the phenotypic variance on average. There were 86 significant locus were identified only under one N condition，which might due to that N supply condition changed expression or regulation some of genes.
