Abstract：Substrates were treated with various amounts of Bacillus amyloliquefaciens，Glomus Mosseae and a commercial bio-fertilizer called Bio twice prior to sowing and after transplanting，in order to figure out the effects of those micro-fertilizers on cucumber（Cacumis sativus L.）root zone environment and yield．The results of 3 constant seasons’ experiments showed that Glomus Mosseae can slow down pH value from raising too fast and can increase EC value in adequate range．Bacillus amyloliquefaciens can significantly decrease the numbers of bacteria，while increase actinomycetes numbers．Bacillus amyloliquefaciens，Glomus Mosseae and Bio can increase root length by 57.1%，51.8% and 44.9%，respectively．They can enlarge root surface areas by 56.5%，51.5% and 44.3%|in addition，all these 3 treatments can increase root volume by 35.4%，82.3% and 61.1%，comparatively．Different micro-fertilizer treatments can improve cucumber plant yield，Bio and GM treatments have remarkable effect on yield increase.
