1Shangqiu Professional Technology College,Shangqiu 476005,Henan,China|2Key Laboratory of Protected Horticulture Co-established by Liaoning Province and Ministry of Education,College of Horticulture,Shenyang Agricultural Uuiversity,Shenyang 110866,Liaoning,China
Abstract:The main active substance extracted from Sophora flavesecns is flavonoids and alkaloids,determine the inhibitory,study the effect of extracts on mycelium dry weight,toxin contents and the activities of extracelluar enzymes,analyze allelopathic inhibition mechanism.Extracts of S. flavescens showed inhibitory effects on Verticillium dahliae allelopathicly owning to its active substances flavonoids and alkaloids.The inhibitory rate is 100.00% 7 days and 98.28% 9 days after treatment with concentration 4.00 mg?mL-1 flavonoids,the inhibitory rate remained about 90% on the 12th day with the concentration 1.00 mg?mL-1 flavonoids.Lower inhibitory rate was found when treated with the same concentration of alkaloids.The mycelium dry weight,toxin contents and the activities of extracellular enzymes decreased with the increase of S. flavescens extracts concentration.The inhibitory effects were significant compared to the CK with the extract concentration over 10 mg?mL-1.By reducing the activities of extracellular enzymes and the toxin content,the growth of mycelium was inhibited,mainly expressed as the reduced dry weight of mycelium.