Abstract：PCR technique was used to detect the bacterial fruit blotch infected seed in Cucurbita pepo L.，and the effect of different treatments including the solution of CuSO4，bleaching powder，HCl，peroxyacetic acid，streptomycin，doxycycline，high temperature on seed disinfection were analyzed．The results showed that the disinfecting solution of CuSO4 and bleaching powder were effective in extinguish the bacterial from seeds．0.5% solution of bleaching powder with 5 minutes can effectively disinfect the bacterial seeds．It is not necessary to get rid of the reagents with water after treating in bleaching powder solution，because the reagent does not affect the seed germination rate and germination energy．Higher temperature treatment can not work well in bacterial fruit blotch extinguishing．
