中国蔬菜 ›› 2012, Vol. 1 ›› Issue (10): 36-41.

• 试验研究 • 上一篇    下一篇

橘红色形态标记在抗根肿病橘红心大白菜雄性不育系转育中的应用

耿新翠,沈向群,李茜,陈永浩,郭艳峰   

  1. 沈阳农业大学园艺学院,辽宁沈阳 110866
  • 收稿日期:2011-12-14 出版日期:2012-05-15 发布日期:2012-02-07
  • 通讯作者: 沈向群,男,研究员,博士生导师,专业方向:蔬菜和花卉遗传育种,E-mail:shenxiangqun54@163.com
  • 作者简介:耿新翠,女,博士研究生,专业方向:蔬菜遗传育种,E-mail:gengxincui@163.com
  • 基金资助:

    农业部项目(201003029)

Orange Morphological Marker’s Applications in Transferring Male Sterile Lines of Orange Head Chinese Cabbage with Anti-clubroot Genes

GENG Xin-cui,SHEN Xiang-qun*,LI Qian,CHEN Yong-hao,GUO Yan-feng   

  1. Department of Horticulture,Shenyang Agricultural University,Shenyang 110866,Liaoning,China
  • Received:2011-12-14 Online:2012-05-15 Published:2012-02-07

摘要: 橘红心大白菜球心色由1对隐性等位基因控制,其子叶颜色、花色的遗传与球心色的遗传表现一致,可以作为橘红心大白菜的形态标记性状在转育中使用。本试验在转育抗根肿病橘红心大白菜核基因雄性不育系的过程中比较了球心色法、子叶法和花色法在不同条件下筛选抗根肿病橘红心雄性不育系的特点。结果表明:无论是种子春化处理还是非种子春化处理,子叶法在子叶展开时就可以筛选到橘红心性状,得到抗根肿病橘红心植株需要抗性筛选的株数最少,耗用根肿菌菌土量最少。可以说,子叶法转育抗根肿病橘红心雄性不育系适用范围广、工作强度小,是3种方法中最理想的方法,利用此方法可以简化抗根肿病橘红心大白菜雄性不育系的转育进程。

关键词: 大白菜, 橘红色, 根肿病, 雄性不育

Abstract: The flower color and cotyledon color could be as morphological marker traits used in transferring orange leaf head Chinese cabbage〔Brassica campestris L.ssp. pekinensis(Lour)Olsson〕for their heredity agreed on that of leaf head color of orange leaf head Chinese cabbage.This color was controlled by a pair of recessive alleles.In this paper,the methods of leaf head color,cotyledon color and flower color were compared during the course of transferring the male sterile lines of orange leaf head Chinese cabbage with anti-clubroot genes under different conditions.When cotyledons were just unfolded,orange leaf head plants were found and picked out with the help of cotyledon color method in situations of seed vernalization or non-seed vernalization survey.Compared with other 2 methods,when screening clubroot-resistant plants,the amount of plants needed to identify were the least by means of cotyledon color method,so did the amount of Plasmodiophora brassicae.Therefore,for it had a wider range of applications and less work intensity,the method of cotyledon color were optimal among these 3 methods.It might be used in breeding resistant varieties or male sterile lines of orange leaf head Chinese cabbage.

Key words: Chinese cabbage, Orange, Clubroot, Male sterility

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