Cloning and Sequence Analysis of CP Gene of Shallot Yellow Stripe Virus Isolated from Welsh Onion in Beijing Area
TIAN Bao-hua1，2，WANG Yong-qin1*，WANG Chan1，2
1Beijing Vegetable Research Center，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100097，China|2School of Life Science，Shanxi University，Taiyuan 030005，Shanxi，China
Abstract：Welsh onions infected by Shallot yellow stripe virus（SYSV）were taken as experiment materials．All contained SYSV-CP coding sequence，partial sequence of NIb protein gene 5′end and 2 gene fragments of 3′UTR were obtained by reverse transcription-polymerase chain reaction（RT- PCR）．They were named as SYSV-CP1 and SYSV-CP2，respectively．The base numbers of their coding region were both 774 bp，encoding 258 amino acids．In this coding region，2 alkali bases were different. In encoding protein，only one amino acid was different．Phylogenetic analysis showed that SYSV isolates can be divided into 2 major groups．SYSV-CP1 and SYSV-CP2 were in the second group．They had the closest relation with the isolates of SYSV from Zhejiang，representing certain geographical distribution correlation．
