（1College of Plant Protection，2College of Resources & Environment，3College of Bioscience and Biotechnology，Hunan Agricultural University，Changsha 410128，Hunan，China；4School of Agronomy，Yunnan University，Kunming 650091，Yunnan，China）
Abstract：Adopting amoA gene clone library construction method，this paper studied the diversity of ammonia-oxidizing bacteria（AOB） and ammonia-oxidizing archaea（ AOA）and correlations between diversity index and urease activity in red soil vegetable field by different fertilization including decomposed organic fertilizer （DNPK），fresh organic fertilizer（FNPK），Nitrogen，Phosphorus and Potassium chemical fertilizer（NPK），Phosphorus and Potassium chemical fertilizer（PK）．The results showed that OTU quantity，Shannon index of AOB library and AOA library represented organic fertilizer（ DNPK and FNPK） were higher than that in chemical fertilizer soil（ NPK and PK）．The dominant AOB groups in FNPK and DNPK treated soil were Nitrosolobus multiformis，which represented 88.5% and 68.5%，respectively．The main AOB groups in chemical fertilizer soil（ NPK and PK） were Nitrosospira sp．with proportion of 54.8% and 65.5%，respectively．The main AOA groups in red earths were Candidatus Nitrosotalea devanaterra，which occupied 90.9% in DNPK treated soil，84.4% in FNPK treated soil，77.8% in NPK treated soil，and 45.2% in PK treated soil．The urease activity
and diversity index in soils treated by DNPK and FNPK were higher than that treated by NPK and PK．There was positive correlation between diversity index of AOA community and urease activity，but there was no marked positive correlation between diversity index of AOB community and urease activity．In conclusion，organic fertilizer was more able to improve the diversity of AOB and AOA communities in red soil of vegetable field than
chemical fertilizer．AOA community played more important role in the process of ammoxidation in red soil of vegetable field．
周志成, 罗 葵, 唐前君, 荣湘民, 刘 强, 何飞飞. 不同施肥方式对红壤蔬菜田氨氧化细菌和氨氧化古菌群落的影响[J]. 中国蔬菜, 2015, 1(7): 33-.
ZHOU Zhi-Cheng, LUO Kui, TANG Qian-Jun, RONG Xiang-Min, LIU Qiang, HE Fei-Fei. Effect of Different Fertilization on Ammonia-oxidizing bacteria and Ammoniaoxidizing archaea in Red Soil Vegetable Field. , 2015, 1(7): 33-.
