中国蔬菜

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不同夜温对茄子套管嫁接苗愈合的影响

赵渊渊1 董春娟1 赵建忠2 尚庆茂1*   

  1. 1 中国农业科学院蔬菜花卉研究所,农业部园艺作物生物学与种质创制重点实验室,北京 100081;2 山西
    省农业种子总站,山西太原 030006
  • 收稿日期:2014-09-25 修回日期:2014-12-10 出版日期:2015-09-28 发布日期:2015-10-05
  • 通讯作者: 尚庆茂,研究员,博士生导师,主 要从事蔬菜苗期发育调控生理及分子生物学方面的研究,E-mail: shangqingmao@caas.cn
  • 作者简介:赵渊渊,硕士研究生,主要从事蔬菜工厂化育苗方面的研究,E-mail: zhaoyuanyuan2009@yeah.net
  • 基金资助:

    现代农业产业技术体系建设专项(CARS-25),公益性行业
    科技专项(20130314)

Effects of Different Temperature at Night on Healing of Tube Grafted Eggplant Plug Seedlings

ZHAO Yuan-yuan 1,DONG Chun-juan 1,ZHAO Jian-zhong 2,SHANG Qing-mao 1*   

  1. 1 Key Laboratory of Biology and Genetic Improvement of Horticultural Corps,Ministry of Agriculture,Institute of
    Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2 Agriculture Seed General
    Station of Shanxi Province,Taiyuan 030006,Shanxi,China
  • Received:2014-09-25 Revised:2014-12-10 Online:2015-09-28 Published:2015-10-05

摘要:

以茄子砧木品种茄砧1 号和接穗品种快圆茄为试材,采用显微观察等方法,研究不同夜温(19、24、29 ℃)对茄
子套管嫁接苗愈合速率及质量的影响。结果表明:在29 ℃昼温条件下,随着夜温的升高,茄子嫁接苗接合部愈伤组织细胞
层数增加,接合部直径增大,但维管束连接及愈合进程减缓,木质部输导能力减弱,愈合质量下降。夜温为19 ℃的处理,
嫁接后第13 天隔离层完全消失,维管束连接,愈合进程较快,木质部输导能力比夜温29 ℃处理提高了72.89%;夜温为29
℃的处理,嫁接后前9 d 愈伤组织产生较多,接合部直径明显增大,比夜温19 ℃处理提高了13.35%,但愈合进程较慢。综
上,在昼温29 ℃的情况下,夜温19 ℃最有利于茄子嫁接苗的愈合。

关键词: 夜温, 茄子, 嫁接, 愈合, 组织细胞学

Abstract:

Taking eggplant(Solanum melongena L.)‘Qiezhen No.1’as rootstock and‘ Kuaiyuanqie’as
scion,this experiment designed treatments with different temperatures(19 ℃,24 ℃,29 ℃)at night to study
the effect of different night temperature on the rate and quality of healing in tube grafted eggplant seedlings by
microscopic inspection.The results showed that the layers of callus cells were increased and the diameters of
joint part were enlarged.While,the process of vascular connection and healing was slowed down,the xylem
translocation ability was weakened,and the quality of healing was deteriorated.The isolation layer completely
disappeared,and vascular was connected on the 13th day after grafting under treatment with 19 ℃ at night.Compared
to the treatment with 29 ℃,the xylem translocation ability was improved by 72.89%.Under treatment with
29 ℃ at night more callus was observed on the 9th day,the diameter of joint part was significantly increased by
13.35%,compared to the treatment with 19 ℃ at night,but the healing process was slower.In conclusion,
under the condition of 29 ℃ day temperature,19 ℃ is the best night temperature for healing of tube grafted
eggplant.

Key words: Temperature at night, Eggplant, Grafting, Healing, Histocytology