(1Dalian City Academy of Agricultural Sciences, Dalian 116036, Liaoning,China;2College of Plant Protection,Shenyang Agricultural University, Shenyang 110161, Liaoning,China;3 College of Life Science, Dalian Nationalities University, Dalian 116600, Liaoning,China)
16S rDNA-PCR-DGGE was used to analyze the bacterial community dynamic changes in rhizosphere soil infected by cucumber Fusarium wilt. There are 5 rhizosphere soil samples, including pre-planting soil, pre-inoculation soil, diseased cucumber and healthy cucumber after inoculation, not inoculated as the control. Total DNA was directly extracted and amplified with F338-GC and R518 primers targeting the 16S rDNA
V3. The amplified were analyzed by DGGE analysis. The result revealed that there was a high diversity of bacterial community compositions among different tested soil samples. Fusarium caused a great bacterial species and number changes in rhizosphere soil, of which Rhodococcus phenolicus, Clostridium sp. and 3 uncultured bacteria increased, another 2 uncultured bacteria reduced after inoculation.