Abstract：Eggplant stem segment tissue culture was easy to be contaminated. Three types of eggplant were selected to test the sterilization effects of 15 kinds of sterilization methods. The best sterilization method was judged according to the contamination rate and plant regeneration rate. The result indicated that different eggplant types were applicable to different sterilization methods. Purple round eggplant was suitable for sterilization method Ⅶ，which was soaked for 10 minutes in 2% NaClO before soaking for 10 minutes in 0.1% HgCl2. Purple long eggplant was suitable for sterilization method Ⅻ，which was soaked for 10 minutes in 4% NaClO before soaking for 10 minutes in 0.3% HgCl2. Green eggplant was suitable for sterilization method ⅩⅢ，which was soaked 10 minutes in 6% NaClO before soaking for 10 minutes in 0.1% HgCl2. The green eggplant stem segment storage experiment showed that low temperature storage could reduce contamination rate. The optimum storage condition was under 5 ℃ for 3 days，the contamination rate remained below 20% and regeneration rate was kept in about 60%. All these could meet the requirements of plant regeneration.