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蚕豆种质资源种子表型性状精准评价

杨生华1 刘 荣2 杨 涛2 张红岩2 杜萌莹2 宗绪晓2*   

  1. 1 临夏州农业科学院,甘肃临夏 731100;2 中国农业科学院作物科学研究所,北京 100081
  • 出版日期:2016-09-26 发布日期:2016-09-26

Accurate Evaluation on Seed Phenotypic Traits of Faba Bean Germplasm Resources

YANG Sheng-hua1,LIU Rong2,YANG Tao2,ZHANG Hong-yan2,DU Meng-Ying2,ZONG Xu-Xiao2*   

  1. 1Linxia Prefecture Academy of Agricultural Sciences,Linxia 731100,Gansu,China;2Institute of Crop Science,
    Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Online:2016-09-26 Published:2016-09-26

摘要:

为深入了解蚕豆种质资源种子表型性状特征及相关性,以防虫网隔离纯化1 代的554 份国内外蚕豆种质资源为试材,
利用相关分析、主成分分析、聚类分析等方法,对采用数码考种仪获得的种子表型性状进行了综合分析。结果表明:供试蚕
豆种质资源种子表型变异范围广,遗传多样性丰富,以百粒重的变异最大;百粒重、种子投影面积、周长、粒长、粒宽和直
径这6 个表型性状之间具有较高的相关性,相关系数均大于0.80 且达到了极显著水平;对8 个数量性状进行主成分分析,
前3 个主成分的贡献率分别为74.60%、23.09% 和1.70%,累计贡献率达到99.39%,几乎覆盖参试种子数量性状的全部信息,
上述6 个表型性状的变异对第1 主成分贡献较大,均为籽粒大小性状;聚类分析将所有参试材料聚为3 类,与播期和地理来
源有一定相关性。基于种子表型性状的综合分析,筛选出11 份具有近圆形种子、适宜全程机械化种植和8 份具有高产潜力
的优异蚕豆资源。

关键词: 蚕豆, 种质资源, 种子表型性状, 相关分析, 主成分分析, 聚类分析, 育种改良

Abstract:

For in-depth understanding the feature and correlation of seed phenotypic traits of faba bean
germplasm resources,comprehensive analyses were conducted in combination with correlation analysis,principal
component analysis and cluster analysis,etc. taking domestic and overseas 554 sets purified for 1 generation
of faba bean germplasm resources by insect nets as material,on seed phenotypic traits obtained from digital
equipment of seed examination. The results revealed that the tested seed phenotypic traits of faba bean germplasm
resources had a wide range variation and rich genetic diversity,especially in the trait of 100-seed weight.
Correlation analysis found that there was high correlation among 6 phenotypic traits of seed,including 100-seed
weight,seed projection area,perimeter,seed length,seed width and diameter;and the correlation coefficients
were greater than 0.80,reaching extremely significant level. The principal component analysis of 8 quantitative
traits showed that the component contribution rate of the former 3 components were 74.60%,23.09% and 1.70%,
respectively,with the cumulative contribution rate of 99.39%,almost covering the whole information of the
quantitative traits of the tested seeds. The variation of the above mentioned 6 phenotypic traits contributed largely to
the first principal component,which were all seed size characters. The cluster analysis showed that all the tested
material were classified into 3 groups associated with sowing time and geographical origin to some extent. Based on
the comprehensive analyses of seed phenotypic traits,11 genotypes with nearly spherical seeds and 8 genotypes
with high yield potential resources were selected for faba bean genetic improvement suitable for mechanized
production,and vegetable faba beans with high yield.

Key words: Faba bean(Vicia faba L.), Germplasm resources, Seed phenotypic traits, Correlation analysis, Principal component analysis, Cluster analysis, Breeding improvement