In order to clarify the effects of Buchnera aphidicola on growth and development of M. persicae in different vegetables，we took vegetables of 3 different families（ radish of Raphanus sativus L.，pepper of Capsicum annuum L.，and cucumber of Cucumis sativus L.） as experimental material，and compared the growth and reproduction situation of M. persicae untreated and treated with Rifampicin．The results showed that the DNAK gene expression quantity of Buchnera in aphids treated with different concentrations of rifampicin was significantly decreased．After treated with 200 μg·mL-1 rifampicin，the reproduction quantity，life span and body weight of aphids were significantly lower than that of the healthy aphids，indicating that endosymbiont Buchnera played a very important role in the growth and development of M. persicae，and the reduction of endosymbiont could have negative effect on M. persicae．