(1Institute of Biology,Hebei Academy of Science,Shijiazhuang 050081,Hebei,China;2Main Crop Disease Microbial Control Engineering Technology Research Center of Hebei Province,Shijiazhuang 050081,Hebei,China;3Hebei University of Technology,Tianjin 300130,China)
Taking Alternaria solani as a target,this study screened the biocontrol strain BAF-6 with
strong antagonism against pathogenic bacteria of potato early blight by plate confrontation method. The strain was
identified as Bacillus amyloliquefaciens through morphological,physiological and biochemical tests and 16S
rDNA sequence analysis. The extracellular metabolites of the strain after 60-80 ℃ heat treatment,its antibacterial
activity was over 95%. Under UV irradiation for 8 hours,or when the pH value was 2-10,its antibacterial
properties were still stable. After protease treatment the strain antibacterial activity was still over 50%.
杨继业,杨帆,崔冠慧,孙劲冲,王雅娜,刘洪伟,程辉彩,张丽萍. 马铃薯早疫病拮抗菌的筛选及其代谢产物的抑菌活性[J]. 中国蔬菜, 2018, 1(5): 62-66.
YANG Ji-ye,YANG Fan,CUI Guan-hui,SUN Jin-chong,WANG Ya-na,LIU Hong-wei,CHENG Hui-cai,ZHANG Li-ping. Screening of Antagonistic Bacteria Against Potato Early Blight and Antibacterial Activity of Its Metabolites. China Vegetables, 2018, 1(5): 62-66.