关键词: 黄瓜, 连作土壤, 填闲作物, 高通量测序, 真菌群落, 细菌群落

Abstract:

Taking the solar greenhouse soil for 3 years successive cucumber cropping as research object，
this paper analyzed the effects of different filling patterns on soil microbial community structure by high-throughput
sequencing technology under successive cucumber cropping．In this study，cucumber was the main crop，
scallion，cole and wheat were the crops for winter and summer leisure period．The results indicated that the
major species in soil bacterial community and fungus community by 3 different filling patterns basically had no
difference，but there were differences in relative abundances among them．At the level of bacteria，among filling
wheat treatment，the relative abundances of Flexibacter，Nitrosomonadaceae_uncultured，naerolineaceae_
unculture were significantly lower than those of scallion and cole．While，the relative abundances of
Flavobacterium，Clostridium and Peptostreptococcaceae_incertae_sedis were significantly higher than those of
scallion and cole．At the level of fungus，among filling wheat treatment，the relative abundances of Ascomycota_
unclassified，Hypocreales_unclassified，Humicola were significantly lower than those of scallion and cole．
While，the relative abundances of Fusarium，Pyrenochaeta，Nectriaceae_unclassified and Preussi were
significantly higher than those of scallion and cole．These results indicated that there were differences in the effects
of different cover crops on soil microbial community structure and diversity．

Key words: Cucumber, Continuous cropping, Cover crops, High-throughput sequencing, Fungal community, Bacterial community