The gynoecious line‘ D1104-2-4’，pistillate line‘ D1158♀-2’ and monoecious line‘ D0528-2’
were used as female parents，and the hybrid generation was obtained by crossing with monoecious line‘ D0708-
2’ as male parent．The paper investigated the female related agronomic characters at 3 periods including
early blooming stage，fruiting early stage and fruiting period； then screened the significantly different combination
to carry out trans-criptional sequence；and finally analyzed the differential genes of MADS-box family among
cucumber hybrid combinations．The results showed that the F1 generation with‘ D1104-2-4’ as female parent
had super parent advantage on the numbers of female flower and female flower node rate．And F1 of all 3 hybrid
combinations had the early maturing mid-parent advantage．The results of transcriptional analysis showed that the
F1 gene expression in the whole gynoecious line‘ D1104-2-4’ combination was significantly biased to the maternal
parent，and only 6 of 37 cucumber MADS-box family genes were different from the female parent，and 17 of them
were differ in expression with male parent．Bioinformatics analyzed and named a significantly differential expre
ss gene CsMADS-box AGL62 that encodes 187 amino acids in the whole length of 745 bp．The encoded protein
was belonged to an unstable hydrophilic transmembrane protein in the mitochondria．The NCBI alignment results
showed that this protein was a transcription factor．Systematic analysis found this protein had the highest homology
to AGL62 in muskmelon．In cucumber，this gene was belonged to the same branch with the other cucumber
AGL62 subtypes and belonged to type I．