Application of Recombinase Polymerase Amplification Technology in Detecting TYLCV
ZHOU Ying1，YANG Li-mei2，LIU Mei1，HUANG Jin-bao1
1 Institute of Plant and Environment Protection，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100097，
China；2 Agricultural Technology Extension Station of Yufa Town，Daxing District，Beijing 102602，China
Tomato yellow leaf curl virus（TYLCV）is an etiology of Tomato yellow leaf curl disease
（TYLCD），which will cause serious damages to tomato production．It is of important significance to exploit rapid
and accurate detection method for controlling this disease．According to DNA-A gene sequence of TYLCV，this
study designed special RPA primers and established a method named TYLCV-RPA，and analyzed the specificity
and sensitivity of this method．The results indicated that TYLCV-RPA method could amplify specific band of 343
bp from sample tomatoes with positive TYLCV．The reaction condition was 37 ℃ for 40 min．This method needed
short time period for amplification with low requirement for equipment．It had no cross reaction with the other
viruses and had good specificity．Its sensitivity was 10 times of the PCR method．It is suitable for rapid detection
of TYLCV．
