中国蔬菜

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硒提高马铃薯幼苗抗寒性生理研究

丁 旭 丁红映 王 明 秦玉芝*   

  1. 湖南农业大学园艺园林学院,湖南长沙 410128
  • 收稿日期:2018-06-26 出版日期:2019-01-01 发布日期:2018-09-03
  • 通讯作者: 秦玉芝,女,博士,副教授,专业方向: 逆境生理与蔬菜遗传育种,E-mail:qyuz@163.com
  • 作者简介:丁旭,本科生,专业方向:园艺学,E-mail:415580812@qq.com
  • 基金资助:

    国家自然科学基金项目(31371683),作物种质创新与资源
    利用重点实验室(省部共建国家重点实验室培育基地)科学基金开放
    项目(15KFXM21)

Studies on Physiology of Cold Tolerance Improved by Selenium in Potato Seedling

DING Xu,DING Hong-ying,WANG Ming,QIN Yu-zhi*   

  1. College of Horticulture and Landscape,Hunan Agricultural University,Changsha 410128,Hunan,China
  • Received:2018-06-26 Online:2019-01-01 Published:2018-09-03

摘要:

低温胁迫是影响我国南方冬闲田马铃薯种植的一大阻碍因素,发生在马铃薯植株生长发育前期。以马铃薯品种金
湘为试材,研究不同硒浓度(1、3、5 mg·L-1)和适宜浓度的喷硒次数(1、2、3 次)对马铃薯幼苗抗寒性的影响。结果表
明:外源喷施1、3、5 mg·L-1 亚硒酸钠处理均可以提高低温胁迫下马铃薯叶片中叶绿素含量,降低叶片相对电导率以及丙
二醛(MDA)、H2O2 含量,且3 mg·L-1 的喷施效果最佳。低温胁迫下喷施3 mg·L-1 亚硒酸钠溶液1~3 次的幼苗叶绿素含
量高于未喷硒对照,MDA 和H2O2 含量均降低,且喷施2 次时效果最好。综上,适宜的喷硒浓度(3 mg·L-1)和次数(2 次)
能在一定程度上降低低温胁迫所带来的伤害,从而提高马铃薯幼苗的抗寒性。

关键词: 马铃薯幼苗, 硒, 低温胁迫, 抗寒生理

Abstract:

Low temperature stress is a major obstacle to potato growth and development especially during
the early stage in winter fallowing fields in southern China.Taking potato variety‘Jinxiang’as test material,
this paper studied the effects of spraying Na2SeO3 with different selenium concentrations(1,3,5 mg·L-1) and
different times(1,2,3)on cold resistance of potato seedlings respectively.The results showed that exogenous
spraying of 1,3,5 mg·L-1 selenium solution could increase the chlorophyll contents of potato leaves,reduce
the relative conductivity,contents of MDA and hydrogen peroxide of leaves under low temperature stress.The
effect of spraying 3 mg·L-1 was the best.The chlorophyll content of seedlings by spraying 3 mg·L-1 selenium
solution for 1-3 times was higher than that of without selenium spraying under low temperature stress,and the
contents of malondialdehyde and hydrogen peroxide were all decreased.The effect of spraying twice was the best.
To sum up,the suitable selenium concentration(3 mg·L-1)and spraying times(twice) can reduce the damage
caused by low temperature stress to certain extent,thus having improved the cold resistance of potato seedlings.

Key words: Potato Seedling, Selenium, Low temperature stress, Cold resistance physiology