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辣椒疫霉病生防细菌的筛选鉴定及其防效

赵旖森 张 亮* 盛 浩 袁 红 周 清   

  1. 湖南农业大学资源环境学院,湖南长沙 410128
  • 收稿日期:2018-04-22 出版日期:2019-01-01 发布日期:2018-10-08
  • 通讯作者: 张亮,男,博士,讲师,硕士生导师, 专业方向:土壤微生物,E-mail:qingzhiweiwu@sina.com
  • 作者简介:赵旖森, 女, 本科生, 专业方向: 农业资源与利用,E-mail: 578228807@qq.com
  • 基金资助:

    国家自然科学基金项目(41571234),湖南农业大学“1515”
    学术创新团队带头人培养项目,湖南农业大学青年科学基金项目,湖
    南农业大学大学生科技创新项目(2017ZK36)

Screening,Identification and Control Effect of a Bio-control Bacteria for Pepper Phytophthora Blight

ZHAO Yi-sen,ZHANG Liang*,SHENG Hao,YUAN Hong,ZHOU Qing    

  1. College of Resource and Environment,Hunan Agricultural University,Changsha 410128,Hunan,China
  • Received:2018-04-22 Online:2019-01-01 Published:2018-10-08

摘要:

以筛选、获取优良辣椒疫霉病害生防菌株材料为目标,通过平板稀释法与对峙法对水稻土壤耕层可培养细菌进行
分离和筛选,成功从167 株可培养细菌中分离、筛选获得1 株拮抗菌LRS-1,并被鉴定为多粘类芽孢杆菌(Paenibacillus
polymyxa
)。该拮抗菌对辣椒疫霉菌具有68% 左右的室内持久抑菌率以及63.4% 的温室病害防效;Realtime-PCR 检测结果表
明该拮抗菌对根围疫霉菌侵染数量具有显著的抑制效果。

关键词: 辣椒疫霉, 拮抗菌, 筛选, 鉴定, 防效

Abstract:

In order to screen and obtain good bio-control bacterial strain,this study isolated and screened
cultivable bacteria from the top paddy soil by means of dilution method and inhibition test in plate. An effective
antagonistic bacteria named LRS-1 was isolated from 167 cultivable bacteria strains. It was identified as
Paenibacillus polymyxa bacteria. The strain owned as much as 68% inhibition rate against Phytophthora capsici
in door and 63.4% controlling effect on Phytophthora blight of pepper in greenhouse. The result of Real-time
PCR test indicated that this strain had remarkable inhibitory effect on Phytophthora pathogen population in
rhizosphere.

Key words: Phytophthora capsici, Antagonistic bacteria, Screen, Identification, Control effect