Investigation and Preliminary Identification of Pathogeny Species of Pepper Virus Disease in Guangxi
GONG Ming-xia1,2,ZHAO Hu1,WANG Meng1,WU Xing1,ZHAO Zeng-jing1,HUANG Jin-mei1,2,#br# HE Zhi1,WANG Ri-sheng1*
(1Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning530007,Guangxi,China; 2Guangxi Key
Laboratory of Vegetable Breeding and New Technology Development,Nanning 53007,Guangxi,China)
Abstract:This paper conducted initial investigation on occurrence situation of pepper virus disease in 9
cities and counties of Guangxi,and identified 8 kinds of common vegetable viruses from 75 suspected infected
pepper samples by double antibody sandwich enzyme-linked immunosorbent assay( DAS-ELISA).The results
showed that the virus diseases occurred in southern and centre Guangxi were generally more severe than in
western and north Guangxi.There were 75 pepper samples reacting positive,among them the infection of
Chilli veinal mottle virus (ChiVMV) was very common.The total detection rate was the highest,reaching
63.38%.It was the prevalent pathogenic virus in Guangxi.The detection rates of Pepper vein mottle virus
(PVMV),Cucumber mosaic virus (CMV),Pepper mild mottle virus (PMMoV) and Chilli ring spot virus
(ChiRSV) were 56.34%,43.66%,35.21% and 33.80%,respectively.Among the 75 positive samples,
87.32% samples were mix-infected by over 2 viruses.Among them,the mixed infections by 2 or 3 viruses
were 32.39% and 29.58%,respectively.Among the mixed infections by 2 kinds of viruses,ChiVMV+CMV
and PVMV+PMMoV were the most common,all holding 21.74%.Among the mixed infections by 3 kinds of
viruses,the mixed infection of ChiVMV+CMV+PVMV was the most common,accounting for 23.81%.