Abstract：This study selected 6 inbred lines of Cucurbita moschata with different salt tolerance as material，analyzed the combining ability of salt tolerance of hybrid progeny by complete double-column hybridization method，and studied the inheritance of salt tolerance of rootstock-used pumpkin．Combining the analysis on each combining ability，we selected relatively excellent preparation combinations from 36 combinations as 18C0077×18C0005，18C0077×18C0046，18C0077×18C0049，18C0024×18C0046，18C0024×18C0049，18C0024×18C0026．At the same time，genetic analysis was conducted on salt tolerance of rootstock-used pumpkin by quantitative trait genetic models，taking the 6 generations（P1、P2、F1、B1、B2、F2） achieved by crossing rootstock-used pumpkin inbred line ‘18C0077’（P1）with strong salt tolerance and rootstock-used pumpkin inbred line‘ 18C0005’（P2） with weak salt tolerance as material．The results showed that the inheritance of salt tolerance of rootstock-used pumpkin was consistent with the ‘2 pairs of additive - dominant - superior main gene+additive - dominant polygene’ model．The genetic rate of main gene of salt tolerance in each generation of rootstock-used pumpkin was 34.62%-62.08%，giving priority to the main gene inheritance，and the heritability of the main gene in B1 was the highest，which was suitable for selection in early generations．
