中国蔬菜

• 专论与综述 • 上一篇    下一篇

马铃薯土传病原菌快速检测方法的比较分析及其应用

李 磊 赵昱榕 谢学文 石延霞 柴阿丽 李宝聚*   

  1. (中国农业科学院蔬菜花卉研究所,北京 100081)
  • 收稿日期:2019-12-02 出版日期:2020-10-01 发布日期:2020-04-29
  • 通讯作者: 李宝聚,男,博士,研究员,专业方向:蔬菜病害综合防治,E-mail:libaoju@caas.cn
  • 作者简介:李磊,男,博士,副研究员,专业方向:蔬菜病害生物防治,E-mail: lilei01@caas.cn
  • 基金资助:
    国家重点研发计划项目(2018YFD020080703),中国农业科
    学院科技创新工程项目(CAAS-ASTIP-IVFCAAS),农业农村部园
    艺作物生物学与种质创制重点实验室项目

Comparative Analysis and Application of Rapid Detection on Potato Soil-borne Pathogens

LI Lei,ZHAO Yu-rong,XIE Xue-wen,SHI Yan-xia,CHAI A-li,LI Bao-ju*   

  1. (Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
  • Received:2019-12-02 Online:2020-10-01 Published:2020-04-29

摘要: 马铃薯土传病害日益严重,对病原菌进行快速检测是病害早期诊断、预测监控、综合防治的重要基础。本文综合比
较了普通PCR、巢式PCR、实时荧光定量PCR、多重PCR 和环介导等温扩增技术(LAMP)在灵敏度和特异性等方面的特点,
以及在检测马铃薯土传病害时的优缺点;总结了各病害检测报道的引物信息和检测体系的优势。同时,对各技术的应用前景
进行展望,旨在对马铃薯土传病害的快速检测方法及其发展趋势有一个全面的了解。

关键词: 马铃薯土传病害, 快速检测, PCR, 灵敏度, 特异性, 综述

Abstract: Potato soil-borne diseases are becoming increasingly serious.Rapid detection on pathogenic
bacteria is an important basis for early diagnosis,predictive monitoring,and comprehensive control of the
diseases.This paper comprehensively compared the characteristics of conventional PCR,nested PCR,realtime
quantitative PCR,multiplex PCR and LAMP technology in sensitivity and specificity,and evaluated their
merits and demerits in detecting potato soil-borne diseases.The paper also summarized the primer information
of various diseases detection reports and advantages of this detection system.At the same time,the paper
prospected the applying future of each technology,aiming at obtaining an overall understanding about the rapid
detection methods and their development tendency on potato soil-borne diseases.

Key words: Potato soil-borne disease, Rapid detection, PCR, Sensitivity, Specificity, Review