中国蔬菜

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栽培茄(Solanum melongena L.)与喀西茄(Solanum khasianum C.B.Clarke)F1杂交种的获得与鉴定

薛金燕 刘益勇 吴隽香 徐照璨 张 宇 成玉富 杨 旭*   

  1. (扬州大学园艺与植物保护学院,扬州大学教育部农业与农产品安全国际合作联合实验室,江苏扬州
    225009)
  • 收稿日期:2019-12-04 出版日期:2020-10-01 发布日期:2020-03-11
  • 通讯作者: 杨旭,男,博士,教授,硕士生导师,专业方向:蔬菜遗传育种与生物技术,E-mail:yangxu@yzu.edu.cn
  • 作者简介:薛金燕, 女, 硕士研究生, 专业方向: 蔬菜分子育种,E-mail: 870181722@qq.com
  • 基金资助:
    国家自然科学基金项目(31972395),扬州大学研究生科研
    创新项目(NXKYCX19-112)

Obtain and Identification of F1 hybrid of Solanum melongena L. and Solanum khasianum C. B. Clarke

XUE Jin-yan,LIU Yi-yong,WU Jun-xiang,XU Zhao-can,ZHANG Yu,CHENG Yu-fu,YANG Xu*   

  1. (School of Horticulture and Plant Protection,Yangzhou University,Agriculture and Agricultural Product Safety International
    Cooperation Joint Laboratory,Yangzhou University,Ministry of Education,Yangzhou 225009,Jiangsu,China)
  • Received:2019-12-04 Online:2020-10-01 Published:2020-03-11

摘要: 喀西茄(Solanum khasianum C. B. Clarke)是茄子近缘野生种,具有珍贵的抵抗生物胁迫及非生物胁迫的基因。以
10 份栽培茄材料作母本与喀西茄进行杂交,并采用一次授粉、重复授粉、花柱短截、混合授粉及嫁接后授粉等授粉方式,
研究获得栽培茄与喀西茄种间杂交种的最佳方法。结果表明:采用嫁接后授粉可以提高杂交结果率,并且得到了L81 与喀西
茄的F1;对F1 苗期植株进行SSR 分子鉴定,证实了F1 种子为栽培茄与喀西茄种间杂交种。

关键词: 喀西茄, 远缘杂交, 嫁接, SSR

Abstract: Solanum khasianum C. B. Clarke is an affinis wild eggplant species,possessing valuable
genes resisting both biotic stress and abiotic stress.Taking 10 cultivated eggplants as female parent,
proceeding hybridization with Solanum khasianum C. B. Clarke,and adopting pollination methods as once
pollination,repeating pollination,style cutting back,mixture pollination and pollination after grafting,
etc.,this study had obtained the best way for interspecies hybridizatyon between Solanum melongena L. and
Solanum khasianum C. B. Clarke.The results showed that adopting pollination after grafting could increase
hybrid fruit setting rate,and obtain F1 hybrid between L81 and Solanum khasianum C. B. Clarke.SSR
molecular identification was conducted on F1 plant at seedling stage and the F1 seed was proven to be the seed of
interspecific hybridization between Solanum melongena L. and Solanum khasianum C. B. Clarke.

Key words: Solanum khasianum C. B. Clarke, Distant hybridization, Graft, SSR