中国蔬菜

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扁豆菌核病病原菌鉴定及其生物学特性

顾玉阳1 王黎娜2 袁 娟3 张 艺1 姚陆铭1 马晓红1 王 彪1* 武天龙1   

  1. (1 上海交通大学农业与生物学院,上海 200240;2 上海红刚青扁豆生产专业合作社,上海201306;3 上海
    市浦东农业技术推广中心,上海201201)
  • 收稿日期:2019-12-30 出版日期:2020-10-01 发布日期:2020-02-21
  • 通讯作者: 王彪,男,副教授,硕士生导师,专业方向:分子生物学,E-mail:wangbiao@sjtu.edu.cn
  • 作者简介:顾玉阳, 男, 硕士研究生, 专业方向: 园艺作物育种,E-mail: guyuyang@sjtu.edu.cn
  • 基金资助:
    上海市科技兴农重点攻关项目〔沪农科创字(2018)第2-4 号〕

Identification of Hyacinth Bean Sclerotinia Pathogen and Its Biological Characteristics

GU Yu-yang1,WANG Li-na2,YUAN Juan3,ZHANG Yi1,YAO Lu-ming1,MA Xiao-hong1,WANG#br# Biao1*,WU Tian-long1   

  1. (1College of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China;2Shanghai Honggang Green
    Hyacinth Bean Production Professional Cooperation,Shanghai 201306,China;3Shanghai Pudong Agricultural Technology
    Extension Center,Shanghai 201201,China)
  • Received:2019-12-30 Online:2020-10-01 Published:2020-02-21

摘要: 近几年,在上海浦东扁豆主产区暴发了一种病害类似于其他作物上发生的菌核病。通过对分离纯化的病原菌进行形
态学观察、致病性鉴定以及分子生物学特征分析,确定了该致病菌就是核盘菌属的核盘菌〔Sclerotinia sclerotiorum(Lib.)de
Bary〕,该菌可以感染扁豆全株,包括根、茎、叶、花、果实都能感染发病,并且能观察到菌核的形成。研究发现扁豆核盘
菌室内分离培养的最佳碳源是果糖,温度22 ℃,pH 为5,适当的逆境生长条件有利于菌核的形成。扁豆、大豆、普通白菜
来源的核盘菌在ITS rDNA 序列上存在差异。将3 种不同来源的核盘菌相比较,发现它们属于不同的融合群,对不同寄主存
在致病能力上的差异,但都能致病。

关键词: 扁豆, 菌核病, 核盘菌, 致病性

Abstract: In recent years,a sclerotinia disease similar to symptoms of the other crops has broken out
at the main hyacinth bean producing area in Shanghai Pudong district.Through conducting mophological
observation,pathogenicity identification on separated and purified pathogenic bacteria,analyzing molecular
biology characteristics,we confirmed that the pathogen causing this disease was Sclerotinia sclerotiorum
(Lib.)de Bary.This pathogen could infect the whole plant of hyacinth bean,including its root,stem,
leaf,flower and pod.We had also observed the formation of its sclerotia.This study discovered that the
best growth carbon source for indoor seperation and culture of Sclerotinia sclerotiorum was fructose at 22 ℃,
pH 5.Proper growth conditions under stress were in favour of forming sclerotia.There were differences
existed in ITS rDNA sequences of Sclerotinia sclerotiorum from hyacinth bean,soybean and pakchoi.
Comparing Sclerotinia sclerotiorum originating from those different sources,we found that those strains
belonged to different fusion groups,and they had discrepant pathogenicities to different hosts,but all of them
could cause diseases.

Key words: Hyacinth bean, Sclerotinia, Sclerotinia sclerotiorum, Pathogenicity