(1College of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China;2Shanghai Honggang Green
Hyacinth Bean Production Professional Cooperation,Shanghai 201306,China;3Shanghai Pudong Agricultural Technology
Extension Center,Shanghai 201201,China)
Abstract:In recent years,a sclerotinia disease similar to symptoms of the other crops has broken out
at the main hyacinth bean producing area in Shanghai Pudong district.Through conducting mophological
observation,pathogenicity identification on separated and purified pathogenic bacteria,analyzing molecular
biology characteristics,we confirmed that the pathogen causing this disease was Sclerotinia sclerotiorum
(Lib.)de Bary.This pathogen could infect the whole plant of hyacinth bean,including its root,stem,
leaf,flower and pod.We had also observed the formation of its sclerotia.This study discovered that the
best growth carbon source for indoor seperation and culture of Sclerotinia sclerotiorum was fructose at 22 ℃,
pH 5.Proper growth conditions under stress were in favour of forming sclerotia.There were differences
existed in ITS rDNA sequences of Sclerotinia sclerotiorum from hyacinth bean,soybean and pakchoi.
Comparing Sclerotinia sclerotiorum originating from those different sources,we found that those strains
belonged to different fusion groups,and they had discrepant pathogenicities to different hosts,but all of them
could cause diseases.