Molecular Marker-assisted Selective Breeding of Sweet Pepper Inbred Line with Resistance Gene(L4)to Tobamovirus and Resistance Gene(Pvr4)to Potato virus Y
Abstract:Tobamovirus is one of the most important viruses harming pepper production in China.In
recent years,damages by pepper mild mottle virus(PMMoV) has become more and more serious.The L allelic genes( L1,L2,L3,L4) are the main resistant genes providing resistance against Tobamovirus.In order
to improve the Tobamovirus and PVY resistance of pepper varieties,this experiment took‘ CM334’( with
resistance to PVY,containing Pvr4 gene)and imported material ‘200375’(with resistantce to PMMoV,
containing L4gene)as resistant resources,and sweet pepper inbred line‘ 83-163’( medium resistance
to Phytophthora blight and CMV)as backcross parents,and conducted multi-generation backcross and
selfing.Utilizing molecular marker-assisted selection(MAS)combined with seedling resistance identification
and morphological selection,this experiment at last obtained the early-maturing,large fruit sweet pepper
selfing line ‘PT83-163’,polymerizing 2 resistance genes L4 and Pvr4,both resistant to Tobamovirus and
PVY(medium resistant to epidemic disease and CMV,containing resistant gene Pvr4 and L4).By artificial
inoculation identification,line‘ PT83-163’ was resistance to disease and its horticultural traits were comparable
to that of the inbred line‘83-163’.