中国蔬菜

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牛蒡根际土壤中解磷菌筛选、鉴定及解磷条件优化

孙 科 耿凤英 于秋菊 王 锋*   

  1. 徐州生物职业技术学院药品食品学院,江苏徐州 221006
  • 收稿日期:2020-03-04 出版日期:2021-01-01 发布日期:2020-04-10
  • 通讯作者: 王锋,男,副教授,专业方向:生 物化工,E-mail:3347489238@qq.com
  • 作者简介:孙科,男,副教授,专业方向:生物发酵技术,E-mail:13912009992@ 139.com
  • 基金资助:
    江苏高校“青蓝工程”资助基金项目(JS1111),徐州市推
    动科技创新项目(KC20051),徐州生物工程职业技术学院科技计划
    项目(2018KY03)

Screening and Identification of Phosphate-solubilizing Microorganism(PSM)in Burdock Rhizosphere Soil and Optimization of Phosphate-solubilizing Conditions

SUN Ke,GENG Fengying,YU Qiuju,WANG Feng*   

  1. Department of Medicine and Food Science,Xuzhou Engineering Vocational and Technical College,Xuzhou 221006,Jiangsu,China
  • Received:2020-03-04 Online:2021-01-01 Published:2020-04-10

摘要: 采用解磷圈法和可溶性磷含量测定法分离筛选出牛蒡根际土壤中解磷能力较强的菌株PSM-5,并对筛选出的菌株
进行解磷条件优化。结果表明:初步鉴定菌株PSM-5 为枯草芽孢杆菌(Bacillus subtilis),优化后最优解磷条件为初始pH 值
3.79、发酵温度28.46 ℃、盐浓度10.46 g · L-1。研究结果为下一步研制牛蒡专用生物有机肥提供解磷菌剂。

关键词: 牛蒡, 解磷菌, 鉴定, 枯草芽孢杆菌

Abstract: Taking phosphate-solubilizing circle method and soluble phosphorus content determination
method,this experiment screened out PSM-5 strain with stronger phosphate-solubilizing ability from Arctium
lappa
L. rhizosphere soil and optimized the phosphate-solubilizing conditions on screened strains.The results
indicated that the strain PSM-5 was tentatively identified as Bacillus subtilis,and after optimization the optimal
conditions for phosphate solubilization were initial 3.79 pH value,28.46 ℃ fermentation temperature and 10.46
g · L-1 salinity.These results have provided phosphate-solubilizing bactericide for next step development of
bio-organic fertilizer dedicated for Arctium lappa L.

Key words: Arctium lappa L. , phosphate-solubilizing microorganism(PSM), identification, Bacillus subtilis