1College of Life Science，Northeast Agricultural University，Harbin 150030，Heilongjiang，China；2College of Horticulture and Landscape Architecture，Northeast Agricultural University，Harbin 150030，Heilongjiang，China
Abstract：CIPK gene family is a critical family in Ca2+ mediated plant signal transduction pathway，which plays a key role in plant stress response and growth．Based on Solanum habrochaites whole genome information，this experiment screened out 24 ShCIPK gene family members，which were divided into 5 subgroups by phylogenetic analysis，and there were 5 gene duplication pairs inside of family members．Subcellular localization analysis showed that most of the ShCIPK genes were located in cytoplasm，while a few were located in plasma membrane，nucleus and mitochondria．The results of multicollinearity analysis indicated that there are 14 and 12 genes in the CIPK gene family of Arabidopsis and cultivated tomato，being collinear with Solanum habrochaites．The result of promoter cis-acting elements prediction analysis identified that ShCIPK family genes contained relevant elements responsive to light，phytohormone，and cold stresses，etc．Real time quantitative PCR analysis showed that the expression levels of ShCIPK2、ShCIPK17、ShCIPK19 were significantly enhanced under low temperature stress，suggesting that these 3 genes might play an important role in the response of Solanum habrochaites to low temperature stress．
柴畅，狄成乾，汪杨，王傲雪. 多毛番茄CIPK基因家族生物信息学分析及低温下功能研究[J]. 中国蔬菜, 2021, 1(2): 47-57.
CHAI Chang，DI Chengqian，WANG Yang，WANG Aoxue. Bioinformatics Analysis of CIPK Gene Family in Solanum habrochaites and Functional Study under Low Temperature. China Vegetables, 2021, 1(2): 47-57.
