Abstract：Recently damages brought by bacterial angular leaf spot to Chinese cabbage in north and
northeastern China has been aggravated year by year．Breeding cultivars resistant to angular leaf spot
has become an important research direction for breeding of Chinese cabbage．But until now a complete
identification method for Chinese cabbage resistance to angular leaf spot has not yet been established．
This experiment carried out isolation and identification on pathogen of bacterial angular leaf spot in Chinese
cabbage planted at the experiment and practice base of Northeast Agricultural University，and confirmed this
pathogen was Pseudomonas syringae pv. syringae van Hall．Taking this isolate as inoculating pathogen and
Chinese cabbage inbred line A224-1 as test material，this experiment adopted orthogonal experiment design of
L9（34），analyzed the effects of inoculation temperature，inoculation humidity and seedling age on occurrence
of bacterial angular leaf spot．The experiment also took inbred line 264-1-1，203-1 and 331-1 as test material
to screen out appropriate inoculating concentration．The results showed that the optimal condition combination
for occurrence of bacterial angular leaf spot in Chinese cabbage was 25-30 ℃ inoculation temperature 90%-
100% inoculation humidity 7-8 leaf-stage seedling age，and optimal inoculation concentration was 1 × 1010
cfu · mL-1．
