Abstract：This paper studied the widely targeted metabolomics on 2 kinds of bottle gourd lines‘I77’and‘Nanxiu’with significant differences in umami taste by technique of combining ultraperformance liquid chromatography-mass spectrometry （UPLC-MS/MS）．Results showed that there were greater differences between metabolites in fruits of different lines of bottle gourd．In total，66 significant different metabolites were screened．Through clustering heat map comparison，fold change（FC）analysis，principal component analysis（PCA）and metabolite pathway analysis on ‘I77’and‘Nanxiu’different metabolites，the results indicated that those metabolites may jointly affect the flavor of bottle gourds，including lipid（9-hydroxy-10，12-octadecadienoic acid，9S-hyroxy-10E，12E-octadecadienoic acid，13-hydroxy-9，
11-octadecadienoic acid，lauric acid，12，13-EODE，lysoPE 14：0），amino acid and its derivatives（oxidized glutathione，L-citrulline，N-acetyl-L-leucine），and flavonoids（luteolin-7-O-rutin）．
