Pathogen Identification of Eggplant Sclerotinia Rot and Screening of Resistant Germplasm Resources
LI Weiliu,WU Yongguan,GAN Guiyun,JIANG Yaqin,YU Ning,LI Wenjia,LI Yan,KANG Dexian,WANG Yikui*
(Institute of Vegetable Research,Guangxi Academy of Agricultural Sciences,Guangxi Key Laboratory of Vegetable Breeding and New Technology Development,Nanning 530007,Guangxi,China)
Abstract:In order to definit the kinds of Sclerotinia rot pathogen and screen out available resources with Sclerotinia rot resistance,this paper separated and purified the collected pathogens,carried out morphology observation and rDNA-ITS sequence analysis,and conducted screen for Sclerotinia rot resistance on 42 eggplant germplasm resources by living stem inoculation method in the fields.The result showed that the pathogen was identified as〔 Sclerotinia sclerotiorum( Lib.) de Bary〕by morphology and molecular biology methods.There were obvious differences in disease resistance of different eggplant germplasm types.Three wild eggplants S. torvum,S. sisymbriifolium and S. anguivi were immune(I),4 of S. aethiopicum were resistant(R) or high resistant(HR),and 4 of Southeast Asian primitive cultivars(S. melongena subsp. ovigerum) were moderate resistant(MR),and all of the advanced cultivars were susceptible(S) or high susceptible(HS).All of the interspecific hybrids were immune(I).Wild relative species of eggplant possessed resistant gene to Sclerotinia rot.This gene can be utilized through hybridization,etc. means.