中国蔬菜 ›› 2022, Vol. 1 ›› Issue (1): 49-57.

• 研究论文 • 上一篇    下一篇

基于基因组结构变异构建萝卜种质资源分子身份证

严承欢1,崔磊1,任志勇1,黄燕1,2,矫振彪1,朱凤娟1,张振兴1,甘彩霞1,邓晓辉1,邱正明1*   

  1. 1湖北省农业科学院经济作物研究所,蔬菜种质创新与遗传改良湖北省重点实验室,湖北武汉 430064;2华中农业大学园艺林学学院,湖北武汉 430070
  • 收稿日期:2021-05-06 修回日期:2021-07-26 出版日期:2022-01-01 发布日期:2022-01-12
  • 通讯作者: 邱正明,男,研究员,主要从事高山蔬菜生态栽培技术研究,E-mail:qiusunmoon@hbaas.com
  • 作者简介:严承欢,男,副研究员,主要从事蔬菜营养品质研究,E-mail:yanch@hbaas.com
  • 基金资助:
    国家重点研发计划项目(2021YFD1600303),国家大宗蔬菜产业技术体系项目(CARS-23-B06),国家自然科学基金项目(31902058),湖北省农业科学院青年科学基金项目(2020NKYJJ04)

Establishment of Molecular Identify Card for Radish Germplasm Resources Based on Genome Structural Variation

YAN Chenghuan1,CUI Lei1,REN Zhiyong1,HUANG Yan1,2,JIAO Zhenbiao1,ZHU Fengjuan1,ZHANG Zhenxing1,GAN Caixia1,DENG Xiaohui1,QIU Zhengming1*   

  1. 1Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement,Institute of Economic Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,Hubei,China;2College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,Hubei,China
  • Received:2021-05-06 Revised:2021-07-26 Online:2022-01-01 Published:2022-01-12

摘要: 萝卜种质资源类型多样,难以通过表型评价判定其亲缘关系。本试验整合4 份萝卜全基因组重测序数据和520 份萝卜简化基因组测序数据进行生物信息学分析,鉴定了萝卜群体基因组水平的结构变异,开发了24 个通用性的分子标记。使用上述标记对255 份萝卜种质进行基因分型,构建了相应的萝卜分子身份证。聚类分析结果表明,在相关系数0.55 处可将255 份萝卜种质分为4 个类群。基于基因组水平的大片段插入/ 缺失开发分子标记,具有通用性高、成本低、检测方便等优点,可以促进萝卜的种质纯度鉴定及分子育种工作。

关键词: 萝卜, 结构变异, 重测序, 分子身份证, 种质资源

Abstract: The types of radish germplasm resources are multiple.It is difficult to determine its genetic relationship by phenotype evaluation.This experiment integrated the whole genome resequencing data of 4 radish accessions and the reduced genomic sequencing data of 520 radish accessions to carry out bioinformatics analysis;identified the structural variation at genome-scale of radish population;and developed 24 user-friendly molecular markers.Using the above markers,this experiment conducted genotyping on 255 accessions of radish germplasm resources,and constructed corresponding radish molecular identity cards.Clustering analysis revealed that 255 accessions radish germplasm resources could be divided into 4 categories at the correlation coefficient of 0.55.Based on the klenow fragment insertion/deletion development of molecular markers at genome level,the advantages of high versatility,low cost,convenient for detection would promote the purity identification of radish germplasm resources and molecular breeding.

Key words: radish, structural variation, DNA re-sequencing, molecular identify card, germplasm resources