Abstract：Cellulose synthase（CESA） is an important enzyme in the process of cellulose synthesis，and plays very important role in cell wall formation and plant growth and development．This experiment identified and gained 35 CESA gene family members from‘9930’genome-wide data of cucumber cultivar，and divided them into 6 groups by phylogenetic analysis．Subcellular localization showed that most of the members were located in the plasma membrane，and a small part was located in the Golgi apparatus．Collinearity analysis of CESA family genes among species showed that there were 15 and 12 homologous gene pairs in the CESA gene families in Arabidopsis and tomato，respectively，and 19 and 20 homologous gene pairs in CESA gene families in watermelon and melon，respectively．Analysis on cis-acting element indicated that these members of CsCESA gene family shared similar cis-acting elements，including plant hormones-responsive，abiotic stressresponsive， temporal and spatial gene expression elements and elements involved in biological processes． Transcriptome data and qRT-PCR results showed that CsCESA gene family were differentially expressed in different tissues，and most of them were induced by abiotic stress and plant hormone．Among them， CsCESA5，CsCESA20 and CsCESA28 could respond to induction of low temperature，salt stress，ABA and MeJA，suggesting that these 3 genes might play an important role in cucumber response to abiotic stress．
