Screening and Identification of Interacting Proteins of Chieh-qua CqWRKY31
YAN Jinqiang 1，2，WANG Min 1，2，PENG Qingwu 1，LIU Wenrui 1，2，JIANG Biao 1，2，XIE Dasen 1，2， HE Xiaoming 1，2*
1 Vegetable Research Institute，Guangdong Academy of Agricultural Sciences，Guangzhou 510640，Guangdong，China； 2 Key Laboratory for Vegetables New Technology Research of Guangdong Province，Guangzhou 510640，Guangdong，China
Abstract：Fusarium wilt is a main disease in chieh-qua production．CqWRKY31 was found as a positive regulator responding to fusaric acid（FA），toxin of Fusarium wilt．In order to excavate the function of CqWRKY31，this experiment screened its interacting proteins by yeast-two-hybrid technology．The results indicated that there were 45 proteins with potential interaction with CqWRKY31．Further verification of rotary experiments confirmed that 42 proteins were interacted with CqWRKY31 in yeast．All positive clones were sequenced and blast analyzed．Among these 42 identified proteins，10 SRC2 or SRC2 homologous proteins， 3 E3 ubiquitin-protein ligase DIS1s，6 DNA-DAMAGE INDUCIBLE proteins and 3 AT-hook motif nuclearlocalized proteins were included．These results had laid a theoretical foundation for analyzing the resistance mechanism of CqWRKY31 in regulating Fusarium wilt of chieh-qua．
