中国蔬菜 ›› 2022, Vol. 1 ›› Issue (10): 58-65.

• 研究论文 • 上一篇    下一篇

南雄辣椒炭疽病菌的分离鉴定与药剂筛选

郑婕1,3,庄远航1,郭梓琪1,许艺1,钟俊杰1,黄维峰1,胡芳1,李海渤1,雷建军1,2*,吴昊1*   

  1. 1韶关学院英东生物与农业学院,广东韶关 512005;2华南农业大学园艺学院,广东广州 510642;3广东省粤北食药资源利用与保护重点实验室,广东韶关 512005
  • 收稿日期:2021-12-02 出版日期:2022-10-01 发布日期:2022-01-25
  • 通讯作者: 雷建军,男,博士,教授,专业方向:蔬菜育种与分子生物学,E-mail:jjlei@scau.edu.cn;吴昊,男,博士,讲师,专业方向:蔬菜分子生物学,E-mail:hwu@sgu.edu.cn
  • 作者简介:郑婕,女,博士,讲师,专业方向:蔬菜分子生物学,E-mail:amanda3049@163.com
  • 基金资助:
    国家自然科学基金项目(32000233),韶关市科技计划项目(210726124532350),南雄市科技特派员项目,2021年韶关学院大学生创新创业训练计划项目(Sycxcy2021148)

Isolation,Identification and Fungicide Screening of Capsicum Anthracnose Pathogen in Nanxiong County

ZHENG Jie1,3,ZHUANG Yuanhang1,GUO Ziqi1,XU Yi1,ZHONG Junjie1,HUANG Weifeng1,HU Fang1,LI Haibo1,LEI Jianjun1,2*,WU Hao1*   

  1. 1Henry Fok College of Biology and Agriculture,Shaoguan University,Shaoguan 512005,Guangdong,China;2College of Horticulture,South China Agricultural University,Guangzhou 510642,Guangdong,China;3Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region,ShaoGuan University,Shaoguan 512005
  • Received:2021-12-02 Online:2022-10-01 Published:2022-01-25

摘要: 为明确广东省韶关市南雄市辣椒炭疽病病原菌种类,并筛选能有效抑制该病原菌的杀菌剂,对采集自南雄市湖口镇露地朝天椒的疑似炭疽病自然发病辣椒果实,利用组织分离法分离纯化炭疽病病原菌,微生物形态学结合多基因系统进化树分析鉴定病菌种类,并通过室内毒力测定试验筛选能够高效防治该病菌的杀菌剂。结果表明,所分离菌株NX-1的病菌种类为Colletotrichumscovillei;12种杀菌剂中,咪鲜胺和苯甲·丙环唑对该病菌的抑制作用最强,EC50分别为0.019 6 μg·mL-1和0.282 8 μg·mL-1。咪唑类杀菌剂咪鲜胺可作为南雄辣椒炭疽病防治药剂的首选,或与苯甲·丙环唑交替使用。

关键词: 辣椒炭疽病, 病原菌鉴定, 室内毒力测定

Abstract: In order to clarify the pathogen species of pepper anthracnose in Nanxiong County,Shaoguan City,Guangdong Province and screen fungicides that can effectively inhibit the pathogen,this study isolated and purified the anthrax pathogen from the fruits of Chaotian pepper with suspected natural anthracnose sympton,collected from open fields of Hukou Town,Nanxiong County by tissue separation method,and the pathogen species were identified by microbial morphology combined with multi-gene phylogenetic tree analysis.Then,fungicides that could efficiently control this pathogen were screened by indoor toxicity test.The results showed that the pathogen of the isolated strain NX-1 was Colletotrichum scovillei.Among 12 fungicides,prochloraz and difenoconazole·propiconazole had the strongest inhibitory effect on the pathogen,with EC50 values of 0.019 6 μg · mL-1 and 0.282 8 μg · mL-1,respectively.Imidazole fungicide prochloraz can be used as the first choice chemist for controlling pepper anthracnose in Nanxiong,or be used alternatively
with benzyl propiconazole.

Key words: capsicum anthracnose, pathogen identification, indoor toxicity measurement