1Henry Fok College of Biology and Agriculture,Shaoguan University,Shaoguan 512005,Guangdong,China;2College of Horticulture,South China Agricultural University,Guangzhou 510642,Guangdong,China;3Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region,ShaoGuan University,Shaoguan 512005
Abstract:In order to clarify the pathogen species of pepper anthracnose in Nanxiong County,Shaoguan City,Guangdong Province and screen fungicides that can effectively inhibit the pathogen,this study isolated and purified the anthrax pathogen from the fruits of Chaotian pepper with suspected natural anthracnose sympton,collected from open fields of Hukou Town,Nanxiong County by tissue separation method,and the pathogen species were identified by microbial morphology combined with multi-gene phylogenetic tree analysis.Then,fungicides that could efficiently control this pathogen were screened by indoor toxicity test.The results showed that the pathogen of the isolated strain NX-1 was Colletotrichum scovillei.Among 12 fungicides,prochloraz and difenoconazole·propiconazole had the strongest inhibitory effect on the pathogen,with EC50 values of 0.019 6 μg · mL-1 and 0.282 8 μg · mL-1,respectively.Imidazole fungicide prochloraz can be used as the first choice chemist for controlling pepper anthracnose in Nanxiong,or be used alternatively
with benzyl propiconazole.