QTL Analysis of Carrot Seed Grain Shape and Thousand-Grain Weight
WANG Xuewei1，OU Chenggang1，HUANG Jianxin2，LIU Xing1，HAO Weidong1，ZHAO Zhiwei1，KONG Xiaoping3，ZHUANG Feiyun1*
〔1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2China Vegetable Seed Technology Co.，Ltd.（Beijing），Beijing 100081；3The Center of Xining Vegetable Service，Xining 810016，Qinghai，China〕
Abstract：Carrot seed grain shape and thousand-grain weight are closely related to seed yield and quality．Taking backcross inbred lines（BILs）colony backcrossed and recombined between a wild carrot species‘Songzi’of distant relative and‘Amsterdam’as test materials，this paper carried out QTL analysis on grain length，grain width，length-width ratio and thousand-grain weight of carrot seeds．The results indicated that the grain length，grain width，length-width ratio and thousand-grain weight of BIL population seed showed a biased normal distribution．There were significant positive correlations between grain length and width and thousand-grain weight．The ratio of length to width was positively correlated with grain length and negatively correlated with grain width，but not with thousand-grain weight，indicating that both grain length and grain width could affect thousand-grain weight．Based on the previously constructed genetic map，3 QTL loci related to grain length，one QTL loci related to grain width and 2 QTL loci related to thousand-grain weight were obtained．Phenotypic contribution rate ranged from 8.8%-18.3%．
