Research on Eliminating of Strawberry Mild Yellow Edge Virus in Fragaria ananassa by Different Heat Treatment Methods
LIU Yufeng1，YAN Zhe1，WANG Zhi1，FENG Linlin2，YU Cui2，REN Junda1*，SHANG Qiaoxia1*
〔1College of Bioscience and Resource Environment，Beijing College of Agriculture，Key Laboratory of Urban Agriculture（North China），Ministry of Agriculture and Rural Affairs，Beijing 102206，China；2Cathay Green Seeds（Beijing）Co.，Ltd，Beijing 102200，China〕
Abstract：The commercially avalable tissue cultured seedlings of strawberry variety‘Benihoppe’were detected by RT-PCR method from 2014 to 2022．It was found that they were mainly infected by strawberry mild yellow edge virus（SMYEV），strawberry vein banding virus（SVBV），strawberry mottle virus（SMoV），strawberry crinkle virus（SCV）and cucumber mosaic virus（CMV）．The total detection rate of positive samples was 20.2%．Only SMYEV and SVBV were detected in tissue cultured seedlings in 2022．Taking the stolons of‘Benihoppe’carrying SMYEV or SVBV virus as test material，this experiment conducted tissue culture．The elimination rate of SMYEV was 25.0% and that of SVBV was 100.0%．In order to further determine the effective method for SMYEV elimination in strawberries，the veneniferous shoot-tip tissue culture seedlings were detoxified by thermotherapy treatment．The virus removal rate was detected by RT-PCR and RTqPCR．The results showed that all 3 thermotherapy treatments could completely（100.0%）eliminate SMYEV from shoot-tip of tissue culture seedlings．Comprehensively considering the survival rate and virus removal rate of tissue culture seedlings，thermotherapy for 30 days after the temperature from 32 ℃ increase 1 ℃ one day to 38 ℃ is the most suitable way for SMYEV elimination．
刘羽丰1，闫哲1，王志1，封林林2，于翠2，任俊达1*，尚巧霞1*. 不同热处理方式脱除草莓植株体内草莓轻型黄边病毒的研究[J]. 中国蔬菜, 2022, 1(11): 92-97.
LIU Yufeng1，YAN Zhe1，WANG Zhi1，FENG Linlin2，YU Cui2，REN Junda1*，SHANG Qiaoxia1*. Research on Eliminating of Strawberry Mild Yellow Edge Virus in Fragaria ananassa by Different Heat Treatment Methods. China Vegetables, 2022, 1(11): 92-97.
