摘要： 农业大学园艺学院，山东青岛 266109；2 中国农业科学院蔬菜花卉研究所，蔬菜生物育种全国重点实验室，北京 100081）
摘 要：株型是影响作物产量和成本投入的重要因素，挖掘株型相关的QTLs 将为黄瓜株型改良提供重要参考。以野生黄瓜PI183967 为母本，以新泰密刺纯系为父本构建了含129 个株系的重组自交系群体，对黄瓜株型的相关性状进行表型调查；同时基于亲本和重组自交系群体的重测序数据构建遗传连锁图谱，进而对黄瓜株型相关性状进行QTL 定位分析。结果表明：基于重测序数据构建了包含598 个bin 标记的遗传连锁图谱，该图谱的遗传总长为1 353 cM，标记间物理距离为
352.8 kb；对株高、节间长度、节位数、侧枝数量和茎粗进行QTL 分析，共定位到7 个株型相关的QTLs，其中株高、节间长度各2 个，节位数、侧枝数量、茎粗各1 个；通过参考注释信息和查阅文献，初步筛选到9 个可能与黄瓜株型相关的候选基因。

关键词: 黄瓜, 株型, 重组自交系, QTL

Abstract: Plant architecture is a significant factor affecting yield and cost input in agricultural production．Mapping QTLs related to plant architecture in cucumber will provide essential reference for genetic improvement of cucumber architecture．In this work，129 recombinant inbred lines which originated from a narrow-cross between cucumber cultivar‘Xintaimici’and wild cucumber‘PI183967’was constructed，and phenotypic investigation related to cucumber architecture was also conducted．At the same time，genetic linkage map was constructed based on resequencing data of parents and recombinant inbred lines，and then QTL mapping analysis related cucumber architecture was performed．The results indicated that the genetic linkage map containing 598 bin markers was constructed，the genetic linkage map’s overall distance is 1 353 cM，and the average physical distance between two bin markers is 352.8 kb．QTL mapping analysis for plant height，internode length，internode number，branch number and Stem diameter was conducted．A total of 7 QTLs were detected，including 2 QTLs for plant height，2 QTLs for internode length，1 QTL for internode number，1 QTL for branch number and 1 QTL for stem diameter．Nine causative genes preliminarily were filtered and predicted by the annotated data and literature．

Key words: cucumber, plant architecture, recombinant inbred lines, QTL