Abstract：In order to clarify the genetic rule of peduncle length of tomato，the four generation populations of P1，P2，F1 and F2 were established from the cultivated variety ‘Ailsa Craig’（AC）with short peduncle length and Solanum lycopersicoides LA1964 with long peduncle length．The major genes and multiple genes mixed genetic model were used to estimate the genetic parameters of tomato peduncle length of the second，third and fourth inflorescences．The results showed that the total peduncle length，anterior peduncle length and posterior peduncle length of the second and third inflorescences were consistent with the MX2-ADI-AD model．But the optimal genetic model for the fourth inflorescence length of total peduncle，anterior peduncle and posterior peduncle was the model of MX2-ADI-ADI．These data indicated that the peduncle length of tomato was controlled by two pairs of dditive-dominant-epistasis major genes + polygenes．It was preliminarily judged that the epistasis effect of polygenes was different due to temperature changes，and the heritability of major genes was 46.61%-77.08%，The heritabilities of polygenes were all 0．These results indicated that the inheritance of major genes played a leading role in the genetic rule of peduncle length of tomato．
