Identification and Bioinformatics Analysis of Heat Shock Protein 20(HSP20)Gene Family in Melon
YANG Senyao1,ZHANG Xiuyue2,MAO Wenwen2,DAI Jin1,GAO Shan1,WANG Dan1,LIU Yongpeng1,HU Jianbin2,LI Lili2*
1Luohe Academy of Agricultural Sciences,Henan Province,Luohe 462000,Henan,China;2College of Horticulture,Henan Agricultural University,Henan Key Laboratory of Fruit and Cucurbit Biology,Zhengzhou 450002,Henan,China
Abstract:Heat shock protein 20(HSP20),an essential protein in the process of plant growth and development,HSP20 plays crucial roles inplants under stress.Based on genomic data of melon(Cucumis melo L.),whole genome identification of the HSP20 gene family was carried out using bioinformatics,and their physical and chemical properties,colinearity,gene structure,evolutionary relationship and cis-elements were analyzed.The results showed that 42 HSP20s were identified in the melon genome data,molecular weight ranging from 8 390.40 to 57 350.14 Da,the intron number of 0 to 12,were unevenly distributed on 12 chromosomes.In colinear analysis,there were 32 genes in melon existing collinearity relation with cucumber,watermelon and pumpkin.Phylogenetic tree reveals 9 pairs of orthologous genes and 8 pairs of paralogous genes of CmHSP20.Multiple cis-elements associated with hormonal response,stress response,photoresponse,and plant development were identified in the promoter of the CmHSP20s gene.
杨森要,张修月,毛文文,戴晋1,高杉,王丹,刘勇鹏,胡建斌,李丽丽. 甜瓜热激蛋白HSP20基因家族鉴定及生物信息学分析[J]. 中国蔬菜, 2023, 1(12): 26-34.
YANG Senyao,ZHANG Xiuyue,MAO Wenwen,DAI Jin,GAO Shan1WANG Dan,Yongpeng,HU Jianbin,LI Lili. Identification and Bioinformatics Analysis of Heat Shock Protein 20(HSP20)Gene Family in Melon. China Vegetables, 2023, 1(12): 26-34.