Effects of Biochar Application on Growth of Pakchoi and Soil Bacterial Community Structure under Microplastic Pollution
WANG Xiying1，MO Zhongmei1，LI Deyan2，ZHANG Lulu1，3，4，ZHAO Hui1，3，4*，TAN Zhiyong1，3，4，HOU Jianwei1
1Tongren University，Tongren 554300，Guizhou，China；2Anshun University，Anshun 561000，Guizhou，China；3Rural Revitalization Research Center of Tongren University，Tongren 554300，Guizhou，China；4Engineering Research Center of Intelligent Monitoring and Policy Simulation for Mountainous Land Space in Colleges and Universities of Guizhou Province，Tongren 554300，Guizhou，China
Abstract：Pot experiments were conducted using‘Sijixiaobaicai’to study the effects of biochar application on the growth of pakchoi on soils contaminated by different microplastics（PP，PE，and PVC）and on soil bacterial community structure and abundance．Functional prediction of soil bacterial community was also conducted．The results showed that microplastic contamination inhibited the fresh mass and leaf number of pakchoi．Biochar application increased the fresh mass，plant height，and root length of pakchoi grown on microplastic-contaminated soils．Co-treatment with microplastics and biochar significantly increased soil bacterial abundance．The highest soil bacterial abundance was found in PER treatment．Co-treatment with PE or PVC and biochar significantly increased the Chao1 index and ACE index of soil bacteria．On the phylum level，Actinobacteria，Proteobacteria，Chlorobacteria，Firmicutes，and Acidobacteria are dominant taxa．Co-treatment with microplastics and biochar increased the relative abundance of Actinobacteria，but decreased the relative abundances of Proteobacteria and Acidobacteria．Soil pH value and organic carbon，ammonium nitrogen，nitrate nitrogen，and available potassium contents were primary influence factors for changes in soil bacterial community structure．Functional prediction of soil bacterial community using PICRUSt showed that biochar application increased the relative abundances of several metabolic pathways of beneficial bacteria，including soil metabolism，environmental information processing，and organic system．To conclude，biochar application had a significant impact on the bacterial community in microplastic-contaminated soils，though the degree of influence varied with the type of microplastics．
王喜英1，莫忠妹1，李德燕2，张露露1，3，4，赵辉1，3，4*，谭智勇1，3，4，侯建伟1. 生物炭对微塑料污染下普通白菜生长和土壤细菌群落结构的影响[J]. 中国蔬菜, 2024, 1(1): 46-59.
WANG Xiying1，MO Zhongmei1，LI Deyan2，ZHANG Lulu1，3，4，ZHAO Hui1，3，4*，TAN Zhiyong1，3，4，HOU Jianwei1. Effects of Biochar Application on Growth of Pakchoi and Soil Bacterial Community Structure under Microplastic Pollution. China Vegetables, 2024, 1(1): 46-59.
