中国蔬菜 ›› 2024, Vol. 1 ›› Issue (1): 60-67.DOI: 10.19928/j.cnki.1000-6346.2023.5060

• 研究论文 • 上一篇    下一篇

几种诱抗剂对西瓜ZYMV的防治效果评价

郝小苑1,刘卓文1,刘枫楠1,刘莉铭1,吴会杰1,古勤生1,康保珊1,2*   

  1. 1 中国农业科学院郑州果树研究所,河南省果树瓜类生物学重点实验室,河南郑州 450009;2 中国农业科学院中原研究中心,河南新乡 453500
  • 收稿日期:2023-07-27 修回日期:2023-09-11 出版日期:2024-01-01 发布日期:2024-01-10
  • 通讯作者: 康保珊,女,博士,副研究员,专业方向:葫芦科作物抗病性遗传,E-mail:kangbaoshan@caas.cn
  • 作者简介:郝小苑,女,硕士,专业方向:西甜瓜病虫害防治,E-mail:hxyuan1998@163.com
  • 基金资助:
    财政部和农业农村部:国家现代农业产业技术体系项目(CARS-25),中国农业科学院科技创新工程项目(CAAS-ASTIP-2022-ZFRI-09),国家自然科学基金项目(31701942)

Evaluation of Several Resistance Inducers’ Control Effects on ZYMV in Watermelon

HAO Xiaoyuan1,LIU Zhuowen1,LIU Fengnan1,LIU Liming1,WU Huijie1,GU Qinsheng1,KANG Baoshan1,2*   

  1. 1Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Henan Key Laboratory of Fruit and Cucurbit Biology,Zhengzhou 450009,Henan,China;2Zhongyuan Research Center,Chinese Academy of Agricultural Sciences,Xinxiang 453500,Henan,China
  • Received:2023-07-27 Revised:2023-09-11 Online:2024-01-01 Published:2024-01-10

摘要: 为探索对西瓜病毒病具有防效作用的植物免疫诱抗剂,以小西葫芦黄花叶病毒(zucchini yellow mosaic virus,ZYMV)为防治对象,植株的显症率及相对防效为指标,评价了6 种不同浓度的哌啶酸(Pip)、N- 羟基哌啶酸(NHP)、没食子酸、苯并噻二唑(BTH)、壳寡糖、β– 氨基丁酸(β-aminobutyric acid,BABA)及其2 种药剂和3 种药剂复配后对该病毒的防治效果。结果显示,单一药剂喷施后对西瓜ZYMV 的防效从高到低依次为:NHP(0.5 mmol·L-1)、Pip(2 mmol·L-1)、壳寡糖(1 g·L-1)、没食子酸(0.5 g·L-1)、BABA(1 mmol·L-1)、BTH(0.1 mmol·L-1);复配药剂试验中,2 种药剂复配防效最好的是2-Ⅶ(NHP 0.5 mmol·L-1 + Pip 2 mmol·L-1),接种后11 d、无菌水对照植株显症率达到100.00%时,该组合处理的植株显症率为10.00%,相对防效达89.99%,但该复配药剂会影响植株生长;3种药剂复配防效较好的组合是3-Ⅰ(没食子酸0.5 g·L-1 + NHP 0.5 mmol·L-1 + BABA 1 mmol·L-1)和3-Ⅱ(没食子酸0.5 g·L-1 + NHP 0.5 mmol·L-1 + Pip 2 mmol·L-1),显症率分别为27.27%、33.33%,相对防效分别为79.49%、68.27%,显著高于其他组合,其中3-Ⅱ处理的植株显症率增加缓慢,防病效果较其他处理持久,防治效果最优。综上,NHP、Pip和没食子酸3种诱抗剂在预防西瓜病毒病害中具有潜在的应用价值。

关键词: 诱抗剂, 西瓜, 小西葫芦黄花叶病毒, 防效

Abstract: In order to explore plant immunoinducer with control effect to watermelon virus disease,taking zucchini yellow mosaic virus(ZYMV)as control object,plant pathogenicity and relative control efficiency as indicators,this paper evaluated 6 agents with different concentrations,including picolinic acid(Pip),N-hydroxy-picolinic acid(NHP),gallic acid,benzothiadiazole(BTH),chitosan,β-aminobutyric acid(BABA),and their two-agent and three-agent combinations for their control effects on this virus disease.The results showed that the control effect of single-agent on ZYMV in watermelon ranked from high to low as follows:NHP(0.5 mmol·L-1),Pip(2 mmol·L-1),chitosan(1 g·L-1),gallic acid(0.5 g·L-1),BABA(1 mmol·L-1),BTH(0.1 mmol·L-1).In the combination agent experiment,the best control effect of the twoagent combination was 2- Ⅶ(NHP 0.5 mmol·L-1 + Pip 2 mmol·L-1).Eleven days after inoculation,when incidence rate of the plant by control-treated water reached 100.00%,the incidence rate of the combinationtreated plants was 10.00%,and relative control effect was 89.99%.However,this combination agent would affect plant growth.The combination of 3 agents with better control effect were 3-Ⅰ(gallic acid 0.5 g·L-1 + NHP 0.5 mmol·L-1 + BABA 1 mmol·L-1)and 3- Ⅱ(gallic acid 0.5 g·L-1 + NHP 0.5 mmol·L-1 + Pip 2 mmol·L-1).Their incidence rates were 27.27% and 33.33%,respectively,and their relative control effects were 79.49% and 68.27%,respectively,significantly higher than those of the other combinations.Among them,the incidence rate of the plants treated with 3-Ⅱ increased slowly,and disease control effect was more lasting than that of the other treatments.Thus,3-Ⅶ had the best control effect on ZYMV.The results indicate that NHP,Pip and gallic acid had potential application value in preventing watermelon virus diseases.

Key words: resistance inducer, watermelon, zucchini yellow mosaic virus, control effect