Molecular Detection of Tomato Spotted Wilt Virus Infected Pepper in Guizhou
TIAN Yu1，LI Chun2，LI Yan1*，WANG Lishuang2*
〔1Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region（Ministry of Education），College of Life Sciences，Institute of Agro-bioengineering，Guizhou University，Guiyang 550025，Guizhou，China；2Institute of Plant Protection，Guizhou Academy of Agricultural Science，Guiyang 550006，Guizhou，China〕
Abstract：Abstract：Tomato spotted wilt virus（TSWV）was identified by RT-PCR in suspected pepper virussamples collected from 6 regions in Guizhou Province，and the N gene sequences of TSWV in different regionswere amplified by molecular cloning．The results showed that TSWV were found in chilies from Guiyang，Anshun，Weng’an，Fuquan，Changshun and Huishui in Guizhou．After sequences aligning，the nucleotideand amino acid identities of the strains isolated from Guizhou were 95.6%-99.7% and 97.1%-100.0%，respectively．Phylogenetic tree analysis suggested that TSWV strains isolated from pepper in Fuquan，Huishuiand Changshun were closely related to those in Yunnan and Shandong．The TSWV strains isolated frompeppers in Anshun，Guiyang and Weng’an were relatively closed to the strains isolated from tomato，pepper，tobacco，celery and Herb of Spanish needles in Yunnan and lettuce in Jiangsu．The study indicated that therewere two TSWV lines in Guizhou pepper，which were certainly correlated with those isolates from varioushosts in different regions in China．
