Transcriptome and Differential Gene Analysis of Pumpkin Material under Waterlogging Stress
LIU Zhenyu1，2，WANG Pengwei1，2，GUO Linxin1，2，CHEN Bihua1，2，SUN Li1，2，LI Xinzheng1，2，LIU Zhenwei1，2*
1College of Horticulture and Landscape Architecture，Henan Institute of Science and Technology，Xinxiang 453003，Henan，China；2Henan Provincial Research Center for Horticultural Plant Resource Utilization and Germplasm Innovation Engineering，Xinxiang 453003，Henan，China
Abstract：In recent years，affected by global climate change，flood disaster happened frequently，has seriously affected the north pumpkin production．To study the mechanism of pumpkin waterlogging tolerance，in this experiment，two pumpkin materials with strong waterlogging tolerance and waterlogging sensitivity were selected as the research objects．Using the double pot method for flooding stress，the expression level of antioxidant enzymes was determined by qRT-PCR，transcriptional sequencing was performed by RNA-seq，and the differentially expressed genes were screened and analyzed for functional annotation．The results were as follows：after flooding stress，the relative expression level of oxidase gene in leaves of waterlogging tolerant material‘013-2’was generally up-regulated，and all of them were higher than that of waterlogging sensitive material‘367-2’．Transcriptome sequencing showed that 3 231 differentially expressed genes were identified in ‘013-2’ and ‘367-2’ leaves，including 1 778 up-regulated genes and 1 453 down-regulated genes．GO enrichment showed that differentially expressed genes were significantly enriched in photosynthesis，ethylene reaction，phosphoric acid signal transduction system，cell response to ethylene stimulation，ethylene activation signal pathway and other biological processes．KEGG annotation revealed that 947 differentially expressed genes were annotated in 121 metabolic pathways，which were significantly enriched in photosynthesis - antenna protein，plant hormone signal transduction，MAPK signal pathway-plant，plant - pathogen interaction，phenylpropane biosynthesis and other metabolic pathways．KEGG GSEA found that plant hormone signal transduction，circadian rhythm-plant，MAPK signal pathway-plant，plant - pathogen interaction and other gene sets scored the highest．KEGG GSEA found that genes related to plant hormone signal transduction，circadian rhythm，MAPK signaling pathway，and plant pathogen interaction received the highest scores．This study laid a foundation for further exploration of regulatory and functional genes related to pumpkin waterlogging tolerance．
刘珍宇1，2，王鹏伟1，2，郭林鑫1，2，陈碧华1，2，孙丽1，2，李新峥1，2，刘振威1，2*. 淹水胁迫下中国南瓜转录组及差异表达基因分析[J]. 中国蔬菜, 2024, 1(5): 105-114.
LIU Zhenyu1，2，WANG Pengwei1，2，GUO Linxin1，2，CHEN Bihua1，2，SUN Li1，2，LI Xinzheng1，2，LIU Zhenwei1，2*. Transcriptome and Differential Gene Analysis of Pumpkin Material under Waterlogging Stress. China Vegetables, 2024, 1(5): 105-114.
