QTL Mapping and Candidate Gene Analysis of Cucumber Immature Fruit Skin Color
1College of Horticulture，Qingdao Agricultural University，Qingdao 266000，Shandong，China；2State Key Laboratory of Vegetable Biobreeding，Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China
Abstract：Abstract：The skin color of immature fruit is an important external trait of cucumber （Cucumis sativus L.）．An F2 population was constructed with the cucumber green immature fruit color material CSIVF0254 and whiteimmature fruit color material CSIVF0021 as the parental lines to study the inheritance pattern and gene mappingof the cucumber immature fruit pericarp color genes．The results showed that green immature fruit color wasincompletely dominant to white immature fruit color，and the major loci Qcsc3.1 and Qcsc5.1 were repeatedlydetected in the spring and autumn of 2022．Qcsc3.1 was localized between markers InDel3842-InDel4076 onchromosome 3，with the LOD value of 17.19 and 24.92，and contribution rates of 36.38%-39.86%．Qcsc5.1 was mapped between markers InDel2342-SSR06660 on chromosome 5，with the LOD value of 13.51 and23.11，and contribution rates of 28.53%-34.19%，respectively．Five genes were predicted via biologicalinformation analysis and qRT-PCR analysis．The results of this study can lay a good foundation for the finemapping of genes regulating fruit skin color and molecular marker-assisted selection and breeding．
