Effi cient Regeneration System for Brassica olerecea Based on Epicotyl Explants
HAN Xichao1，2，ZHANG Xiaojian2，CAI Xu2，LIANG Jianli2，WANG Xiaowu2，ZHU Keming1*，WU Jian2*
1College of Life Sciences，Jiangsu University，Zhenjiang 212013，Jiangsu，China；2Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，State Key Laboratory of Vegetable Biobreeding，Beijing 100081，China
Abstract：The hypocotyl is a commonly used explant for genetic transformation of cabbage（Brassica oleracea var. capitata），however，the problems of genotype-dependent and low regeneration efficiency are the main obstacles at present．This study explores the construction of a genetic transformation system using the epicotyl of cabbag e as an explant，with 9 cabbage varieties as test materials．The regeneration effi ciency after Agrobacterium infection was compared between the hypocotyl and epicotyl as two explants．The results show that 5 cabbage varieties had elongated epicotyls．After Agrobacterium inoculation，all 5 varieties with epicotyls as explant induced adventitious shoots with regeneration frequencies ranging from 32.4% to 78.4%．In contrast，only 3 varieties induced of adventitious buds with hypocotyl as explant，with the highest regeneration frequency of only 2.7%．This shows that the epicotyl is an ideal explant for overcoming genotypic dependence in B. olerecea transformation，and provides an effi cient approach to obtain regenerated B. oleracea plants．
韩昔潮，张肖健，蔡旭，梁建丽，王晓武，朱克明，武剑. 基于上胚轴外植体的甘蓝高效再生体系[J]. 中国蔬菜, 2024, 1(9): 44-50.
HAN Xichao，ZHANG Xiaojian，CAI Xu，LIANG Jianli，WANG Xiaowu，ZHU Keming，WU Jian. Effi cient Regeneration System for Brassica olerecea Based on Epicotyl Explants. China Vegetables, 2024, 1(9): 44-50.
