Diversity Analysis of 72 Purple-orange Chinese Cabbage Germplasm Resources
GONG Ying,BAO Yongrong,HUO Jianyu,ZHANG Lugang*,NIE Shanshan*
State Key Laboratory of Crop Stress Resistance and High-Efficiency Production,College of Horticulture,Northwest A&F University,Yangling 712100,Shaanxi,China
Abstract:In order to breed new varieties of purple-orange Chinese cabbage and enrich the variety types of Chinese cabbage,the genetic diversity and group division of 72 purple-orange Chinese cabbage inbred lines were analyzed based on phenotypic characters and SSR molecular markers.The results showed that 72 purpleorange Chinese cabbage inbred lines were divided into 4 groups by phenotypic clustering,and each group separately contained 13-24 germplasm,indicating that the morphological types of purple-orange Chinese cabbage inbred lines were rich and evenly distributed in each group.The average Nei’s gene diversity index and Shannon’s diversity information index by SSR molecular marker were 0.274 5 and 0.419 5,respectively,which indicated that the genetic background of purple-orange Chinese cabbage inbred lines was relatively simple.SSR marker clustering divided 72 purple-orange Chinese cabbage inbred lines into 5 groups and 2 independent germplasm,with each group containing 2-40 germplasm,and the germplasm distribution among groups was uneven.The results of SSR marker could cluster most germplasm with the same pedigree sources into the same group.Kappa consistency test showed that the clustering results of phenotypic traits were not consistent with those of SSR markers,which was a result of phenotypic traits were inconsistent with SSR markers in representativeness,coverage and correspondence.