Abstract：Pathogenesis-related proteins（PRs）were important defense protein of plants，induced in
responses to diff erent environmental stresses．PR-17 was a new member of the PRs family．In this study，CsPRp27 was cloned from resistance cucumber D1322 leaves by reverse transcription-polymerase chain reaction（RT-PCR）and its bioinformatics was analyzed．Results showed that the length of open reading frame of CsPRp27 was 750 bp and encoded 260 amino acids，belonged to member of basic secretory proteins family．CsPRp27 was a stable，basic，secretory protein，and contained a transmembrane domain between 37th and 59th amino acids．Phylogenetic tree analysis showed that CsPRp27 and CmPRp27（melon）falled into the same branch，and had close genetic relationship with Nicotiana tabacum，Solanum tuberosum，Capsicum annuum and Vitis vinifera，which were had similar protein motif 1-motif 5．According to promoter sequence and interaction protein prediction，expression of CsPRp27 probably regulated by MeJA and histone modification．The result was a foundation for further exploring the function of CsPRp27 in cucumber in resistance to plant disease.
