Cloning of Encoding FMRFamide-like Peptide (Mi-flp-16)Gene in Meloidogyne incognita and Analysis of in Situ Hybridization
WANG Tian-yang1，XIAO Luo2，CHEN Guo-hua1，MAO Zhen-chuan1，YANG Yu-hong1,XIE Bing-yan1*
1Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences，Beijing 100081,China；2College of Biosafety Science and Technology,Hunan Agricultural University，Changsha 410128,Hunan,China
Abstract：To clone and characterize the encoding FMRFamid-like peptides genes from Meloidogyne incognita,total RNA was extracted from the J2 nematodes.One gene was obtained by RACE and named Mi-flp-16(GenBank：EU549831).The full length cDNA sequence of Mi-flp-16 was 690 bp,with a 528 bp ORF and encoding a 176 amino acids protein.The protein has three FLPs,including two AQTFVRF and one GQTFVRF.Using in situ hybridization technique to detect the expression of Mi-flp-16,found that it was expressed in neurones associated with the circumpharyngeal nerve ring.
