Research Progress on Agrobacterium tumefaciens Mediated Genetic Transformation of Watermelon
LI Juan1，LI Wan-ning2，TANG Yi1，LI Huan-xiu1*
1College of Horticulture，Sichuan Agricultural University，Ya’an 625014，Sichuan，China；2Rural Development Bureau of Shuangliu County，Shuangliu 610200，Sichuan，China
Abstract：The paper introduces the present status of studies on Agrobacterium tumefaciens- mediated genetic transformation of watermelon〔Citrullus lanatus（Thunb.）Matsum. et Nakai〕，summarizes the major influencing factors in establishing efficient regeneration system of watermelon and genetic transformation process．The best selection of explants，the optimal medium preparation，and antibiotics，types of Agrobacterium tumefaciens，concentration of bacteria，infecting time，pre-culture time，culture time and add acetyl clove ketone，etc are summed up．The paper also discusses issues existing in watermelon genetic transformation and prospects the future development of this system.
